New Tech's automatic nucleic acid m'zigawo ndi kuzindikira makina

Mphamvu zisanu:
● Chida chopangidwa ndi nucleic acid m'zigawo ndi sitepe yoyeretsedwa
● Chida kukhazikitsidwa ndi akupanga m'zigawo gawo
● Chida chokonzedwa ndi zodziwikiratu
● Chida chokhazikitsidwa ndi kukulitsa kutentha kosiyanasiyana
● Chida chokonzedwa ndi zida zotsekera bwino

1.Kodi ma nucleic acid reagents ayenera kuchotsedwa ndikuyeretsedwa?
Mfundo yozindikiritsa ma nucleic acid ndi motere: pansi pa zoyambira, DNA polymerase imagwiritsidwa ntchito popanga ma chain reaction amplification pa template DNA/RNA (yofunikira kusindikiza kwa NA), ndiyeno kuchuluka kwa siginecha ya fulorosenti yomwe imatulutsidwa imadziwika kuti idziwe. ngati chitsanzocho chili ndi nucleic acid (DNA / RNA) ya tizilombo toyambitsa matenda kuti tipezeke.

1) Zitsanzo zomwe sizinachotsedwe kapena kuyeretsedwa zimatha kukhala ndi zigawo zambiri zomwe zimakhudza zotsatira zomaliza: nuclease (yomwe imatha kusungunula chandamale cha nucleic acid ndikuyambitsa zolakwika zabodza), protease (yomwe imatha kuchepetsa DNA polymerase ndikuyambitsa zabodza), heavy metal. mchere (womwe umayambitsa kusagwira ntchito kwa synthase ndikuyambitsa zabodza), acidic kwambiri kapena zamchere PH (zomwe zingayambitse kulephera), Zosakwanira RNA (yomwe imatsogolera ku kulephera kwa zolembera zabodza).

2) Zitsanzo zina zimakhala zovuta kukulitsa mwachindunji: Gram-positive ndi majeremusi ena, chifukwa cha makoma awo akuluakulu a cell ndi zina, ngati sadutsa mu nucleic acid m'zigawo ndi kuyeretsa ndondomeko, zida zopanda m'zigawo zingathe kulephera. zitsanzo.

Chifukwa chake, tikulimbikitsidwa kusankha zida zoyesera kapena chida chokhazikitsidwa ndi gawo la nucleic acid.

2. Chemical m'zigawo kapena thupi akupanga kugawikana m'zigawo?
Nthawi zambiri, kuchotsa mankhwala kutha kugwiritsidwa ntchito pamankhwala ambiri komanso Kuyeretsa. Komabe, mabakiteriya a Gram-positive okhala ndi mipanda yolimba komanso majeremusi ena, ndizomwe zimachititsanso kuti kuchotsa mankhwala kungathe kupeza ma templates a nucleic acid, zomwe zimapangitsa kuti anthu azindikire zolakwika. Komanso, mankhwala m'zigawo zambiri amagwiritsa amphamvu wothandizira, ngati elution si bwinobwino, n'zosavuta kuti atchule wamphamvu zamchere mu dongosolo anachita, chifukwa zotsatira zolakwika.

Akupanga kugawikana kumagwiritsa ntchito kuphwanya thupi, komwe kwagwiritsidwa ntchito bwino ndi GeneXpert, bizinesi yotsogola m'munda wa POCT kuti agwiritsidwe ntchito ndi anthu, ndipo ili ndi mwayi wochotsa nucleic acid wa zitsanzo zovuta (monga Mycobacterium tuberculosis).

Chifukwa chake, tikulimbikitsidwa kusankha zida zoyesera kapena chida chokhazikitsidwa ndi gawo lochotsa nucleic acid. ndipo ndi mulingo woyenera kwambiri ngati pali akupanga m'zigawo gawo.

3. Buku, Semi-zodziwikiratu komanso zodziwikiratu?
Ili ndi vuto la ndalama zogwirira ntchito komanso kulimbikira ntchito. Pakali pano, zipatala za ziweto zopanda antchito okwanira, ndi kuchotsa nucleic acid ndi kuzindikira ndi ntchito yomwe imafuna luso ndi chidziwitso. Palibe kukayika kuti makina odziwikiratu a nucleic acid ndi makina ozindikira ndiye chisankho chabwino kwambiri.

4. Kukulitsa kutentha kosalekeza kapena kukulitsa kutentha?
Kachitidwe kakukulirakulira ndi ulalo wodziwikiratu wa nucleic acid, ndipo ukadaulo waukadaulo womwe ukukhudzidwa ndi ulalowu ndiwovuta. Mwachidule, ma enzymes amagwiritsidwa ntchito kukulitsa nucleic acid. Pakukulitsa, chizindikiro cha fluorescence chokulirapo kapena chizindikiro chophatikizidwa cha fluorescence chimadziwika. Nthawi zambiri, chizindikiro cha fluorescence chikawonekera koyamba, ndiye kuti chiwopsezo cha jini chimakhala chokulirapo.

Kuchulukitsa kwa kutentha kosalekeza ndi nucleic acid amplification pa kutentha kokhazikika, pamene kusinthasintha kwa kutentha ndiko kukulitsa kwa cyclic molingana ndi denaturation-annealing-extension. Nthawi yowonjezera kutentha yakhala ikuchitika, pamene nthawi yowonjezera kutentha imakhudzidwa kwambiri ndi kutentha kwa kutentha ndi kugwa kwa chipangizocho (pakali pano, opanga ambiri atha kupanga maulendo a 40 pafupifupi mphindi 30).

Ngati mikhalidwe ya labotale ili yabwino ndipo malowo ndi okhwima, ndizomveka kunena kuti kusiyana kolondola pakati pa ziwirizi sikudzakhala kwakukulu. Komabe, kukulitsa kutentha kosinthika kudzapanga zinthu zambiri za nucleic acid munthawi yochepa. Kwa ma laboratories opanda madera okhwima komanso ogwira ntchito yophunzitsa, chiopsezo cha nucleic acid aerosol chiwopsezo chidzakhala chokulirapo, zabodza zimachitika pakangotayikira, ndipo zomwe zimakhala zovuta kuzichotsa.

Kuphatikiza apo, kukulitsa kutentha kwanthawi zonse kumakhalanso kosavuta kukulitsa kusagwirizana kwenikweni pamene chitsanzocho chili chovuta (kutentha kwachibale kumakhala kotsika, komanso kutentha kowonjezera, kumapangitsanso kutanthauzira koyambirira).

Ponena za ukadaulo wamakono, kukulitsa kutentha kosinthika ndikodalirika.

5. Mungapewe bwanji chiwopsezo cha kutayikira kwa nucleic acid amplification?
Pakalipano, opanga ambiri amasankha gland mtundu wa PCR chubu monga nucleic acid reaction chubu, yomwe imasindikizidwa ndi kukangana, ndi kutentha kwa denaturation mu kusintha kwa kutentha kwa kusintha kwa kutentha kwa PCR kukulitsa kufika pa 90 digiri.
Centigrade . Kuchulukitsa mobwerezabwereza ndi kutentha ndi kutsika ndi kuzizira ndizovuta kwambiri kusindikiza kwa chubu cha PCR, ndipo mtundu wa PCR chubu ya gland ndiyosavuta kuyambitsa kutayikira.

Ndikwabwino kutengera zomwe zimachitikira ndi zida zomata/chubu kuti mupewe kutayikira kwa zomwe zachitika. Zingakhale zabwino ngati pangakhale zida zomata bwino kuti muchotse nucleic acid ndikuzindikira.

Chifukwa chake makina atsopano a New Tech odzipangira okha nucleic acid ndi ozindikira ali ndi zisanu zomwe zili pamwambazi.