Canine Cortisol (cCortisol) ndi mahomoni a steroid omwe amapangidwa ndi canine adrenal cortex.Cortisol imathandizira kukhalabe ndi shuga wambiri m'magazi, imachepetsa kutupa komanso imathandizira thupi kuthana ndi nkhawa.Ngakhale kuti matenda osiyanasiyana obwera chifukwa cha kuchulukirachulukira kwa timadzi ta mowa amatchedwa Cushing syndrome (CS), agalu ndi amphaka amatha kudwala CS, yomwe imapezeka kwambiri mwa agalu kuposa amphaka.Agalu apakati ndi achikulire (zaka 7 mpaka 12)
amadwala matendawa.Matendawa amayamba pang'onopang'ono ndipo zizindikiro zoyambirira zimakhala zovuta kuzizindikira.CS ikhoza kuzindikiridwa ndi adrenocorticotropic hormone (ACTH) test stimulation test and dexamethasone suppression test, ndi mitundu yake yosiyanasiyana: adrenal-dependent (ATH) ndi pituitary-dependent (PDH).
Izi zimagwiritsa ntchito fluorescence immunochromatography kuti zizindikire kuchuluka kwa cCortisol mu seramu ya galu/plasma.Mfundo yofunikira: Mizere ya T ndi C imayikidwa pa nembanemba ya nitrocellulose, mzere wa T umakutidwa ndi cCortisol antigen a, ndipo chomangiracho chimapopera ndi fulorosenti nanomaterial yolembedwa kuti antibody b yomwe imatha kuzindikira cCortisol.
iye cCortisol mu chitsanzo choyamba cholembedwa ndi nanomaterial.Ma antibody B amamangirira kuti apange zinthu zovuta, kenako ma chromatographs kupita mmwamba.Zovutazo zimapikisana ndi T-line antigen a ndipo sizingatheke kugwidwa;M'malo mwake, ngati palibe cCortisol mu zitsanzo, antibody B amamanga ku antigen a.Kuwala kosangalatsa kukayatsidwa, zida za nano zimatulutsa siginecha ya fulorosenti, ndipo mphamvu ya siginoyo imayenderana mosagwirizana ndi kuchuluka kwa cCortisol pachitsanzo.
Chiyambireni kukhazikitsidwa kwake, fakitale yathu yakhala ikupanga zinthu zapamwamba kwambiri padziko lonse lapansi ndikutsatira mfundoyi
za khalidwe poyamba.Zogulitsa zathu zapeza mbiri yabwino pamsika komanso kukhulupirika pakati pa makasitomala atsopano ndi akale..