Feline Health Marker kuphatikiza kuzindikira (5-6 zinthu)

【Cholinga choyesera】
Feline pancreatic lipase (fPL) : Pancreas ndiye chiwalo chachiwiri chachikulu kwambiri cham'mimba mwa nyama (choyamba ndi chiwindi), chomwe chili kumimba yakutsogolo kwa thupi, yogawidwa kumanzere ndi kumanja.ntchito yake yaikulu ndi secrete michere zofunika kwa thupi.Pancreatitis lagawidwa pachimake kapamba ndi aakulu kapamba.Zowonongeka zomwe zimayambitsidwa ndi zakale nthawi zambiri zimakhala zosakhalitsa, pomwe zotsalirazi zimasiya fibrosis yokhazikika ndi atrophy panthawi ya kutupa kosatha.Mwa iwo, kapamba osatha amakhala pafupifupi 2/3 ya kapamba amphaka.
Cholyglycine (CG) ndi imodzi mwa ma conjugated cholic acid omwe amapangidwa ndi kuphatikiza kwa cholic acid ndi glycine.Glycocholic acid ndi gawo lofunikira kwambiri la bile acid mu seramu panthawi yoyembekezera.Maselo a chiwindi atawonongeka, kutengeka kwa CG ndi maselo a chiwindi kunachepa, zomwe zinachititsa kuwonjezeka kwa CG m'magazi.Mu cholestasis, excretion wa cholic acid ndi chiwindi ndi mkhutu, ndipo zili CG kubwerera kwa magazi ndi kuchuluka, amenenso kumawonjezera zili CG mu magazi. kudzera munjira ya chiwindi mutadya.Momwemonso, matenda a chiwindi ndi kutsekeka kwa ma ducts a bile kungayambitse vuto lachilendo.
Cystatin C ndi imodzi mwa mapuloteni a cystatin.Chofunika kwambiri zokhudza thupi ntchito ndi kulamulira ntchito cysteine ​​protease, amene ali amphamvu chopinga zotsatira pa cathepsin B, papain, nkhuyu protease, ndi cathepsin H ndipo ine anamasulidwa ndi lysosomes.Imakhala ndi gawo lofunikira mu metabolism ya ma intracellular peptides ndi mapuloteni, makamaka mu metabolism ya collagen, yomwe imatha kutulutsa ma prehormones ena ndikuwatulutsa m'matenda omwe akufuna kuti agwire ntchito zawo.Cholowa muubongo kukha magazi ndi amyloidosis ndi matenda okhudzana mwachindunji ndi cystatin C gene mutation, omwe angayambitse ubongo kuphulika kwa mitsempha, kutulutsa magazi muubongo ndi zotsatira zina zazikulu.Impso ndi malo okhawo ochotsera cystatin C yozungulira, ndipo kupanga cystatin C kumakhala kosalekeza.Mulingo wa cystatin C mu seramu makamaka umadalira GFR, chomwe ndi cholembera chamkati chomwe chimawonetsa kusintha kwa GFR.Kusintha kwa zomwe zili m'madzi ena amthupi kumalumikizidwanso ndi matenda osiyanasiyana.
NT-proBNP (N-terminal pro-brain natriuretic peptide), yomwe imadziwikanso kuti B-type diuretic peptide, ndi mahomoni opangidwa ndi cardiomyocyte m'magawo a mtima.Kuthamanga kwa magazi kwa ventricular kumawonjezeka, kuchepa kwa ventricular, hypertrophy ya myocardial, kapena kuthamanga kwa myocardial kumawonjezeka, kalambulabwalo wa NT-proBNP, proBNP (yopangidwa ndi 108 amino acid), imatulutsidwa m'magazi ndi cardiomyocytes.
Cat allergen total IgE (fTIgE) :IgE ndi mtundu wa immunoglobulin (Ig) wokhala ndi kulemera kwa 188kD komanso wochepa kwambiri mu seramu.Nthawi zambiri ntchito matenda a thupi lawo siligwirizana.Kuphatikiza apo, ingathandizenso pakuzindikira matenda a parasitic ndi ma myeloma angapo.1. Matupi awo sagwirizana: pamene ziwengo zimachitika, zimabweretsa kuwonjezeka kwa allergen lgE.Kukwera kwa allergener LgE, m'pamenenso kusagwirizana ndizovuta kwambiri.2. Matenda a tizilombo: chiweto chikadwala ndi tizilombo toyambitsa matenda, allergen LgE imathanso kuwonjezeka, yomwe nthawi zambiri imakhala yokhudzana ndi kuchepa pang'ono chifukwa cha mapuloteni a tizilombo.Kuphatikiza apo, kupezeka kwa khansa kungapangitsenso kukweza kwa IgE yonse.
【 Mfundo yodziwira】
Mankhwalawa amagwiritsa ntchito fluorescence immunochromatography kuti azindikire mochulukira zomwe zili mu fPL/CG/fCysC/fNT-proBNP/fTIgE m'magazi amphaka.Mfundo yaikulu ndi yakuti nembanemba ya nitrocellulose imakhala ndi mizere ya T ndi C, ndipo mzere wa T umakutidwa ndi antibody omwe amazindikira makamaka antigen.Pad yomangiriza imapopera ndi fulorosenti ina ya nanomaterial yotchedwa antibody b yomwe imatha kuzindikira antigen.Antibody mu chitsanzocho imamangiriza ku nanomaterial yolembedwa kuti antibody b kupanga zovuta, zomwe kenako zimamanga ku T-line antibody A kupanga masangweji.Kuwala kosangalatsa kukayatsidwa, nanomaterial imatulutsa ma siginecha a fulorosenti.Kuchuluka kwa chizindikirocho kunali kogwirizana bwino ndi ndende ya antigen mu chitsanzo.