Kuchuluka kwa progesterone ya canine mu seramu kumagwirizana ndi gawo la galu estrus.Poyerekeza ndi LH, kuchuluka kwa cProg nthawi zonse kumawonjezeka panthawi ya estrus ya galu wamkazi, yomwe imakhala yosavuta kufufuza ndipo ikhoza kusinthidwa nthawi yeniyeni Nthawi yabwino yobereketsa ndi masiku 3-6 pambuyo pa chiwongoladzanja cha LH, malingana ndi mkazi. mkhalidwe wa estrus wa galu.Pakati pa akazi osiyanasiyana, Mlingo wa progesterone wofanana ndi nthawi yabwino yokwerera umasiyana mosiyanasiyana, nthawi zambiri kuyambira 0-50ng / ml, koma panalinso zambiri kuposa izi, motero, kuchuluka kwa keratinization ya epithelium ya nyini kunaphatikizidwa. Kuyang'anira nthawi yeniyeni ya kuchuluka kwa progesterone mu seramu Njira yowunikira ingathandize kwambiri kuthekera kwa kutenga pakati kwa agalu achikazi.
cProg zomwe zili mu seramu ya galu / plasma zidadziwika mochulukira ndi fluorescence immunochromatography.Mfundo yofunika: Fiber nitrate Pali mizere ya T ndi C pa filimu yowoneka bwino, ndipo mzere wa T umakutidwa ndi cProg antigen a, yomwe imatha kuzindikira cProg mwa kupopera mbewu pa pedi.
cProg pachitsanzocho idalumikizidwa koyamba ndi ma nanomaterial otchedwa antibody b kupanga Complex, kenako mpaka kumtunda, zovutazo zimapikisana ndi T-line antigen a ndipo sizingagwire;M'malo mwake, ngati palibe chitsanzo Pamaso pa cProg, antibody b imamanga ku antigen a.Pamene chisangalalo kuwala walitsa, ndi nanomaterial zimatulutsa fluorescence chizindikiro.Mphamvu ya siginecha imayenderana mosagwirizana ndi kuchuluka kwa cProg pachitsanzo.
Popeza milingo yabwino kwambiri ya progesterone imakhudzana ndi mtundu, zaka ndi kukula kwa galu, palibe mtengo wokhazikika, magawo otsatirawa:
Pongofotokozera kokha, tikulimbikitsidwa kuti labotale iliyonse kapena chipatala chikhazikitse malo ake omwe amalozera malinga ndi chipatala
Osati pa kutentha:<1ng/ml;
Estrus:kuchuluka kwa progesterone kumawonjezeka pang'onopang'ono, kuzungulira kumakhala masiku 7-8;Pofuna kupititsa patsogolo bwino kwa mimba, mayeso oyambirira a progesterone
Mlingo uyenera kukhala mkati mwa 10-50ng / ml, ndipo tikulimbikitsidwa kuswana kawiri.
10-30ng/ml:kukweretsa koyamba mkati mwa 3h, kukweretsa kachiwiri mkati mwa 48h;
30-60ng / ml:kukweretsa koyamba mkati mwa 2h ndi makwerero achiwiri mkati mwa 24h;
60-80ng / ml:2h kwa makwerero.
Kuzindikira kwa zida izi ndi 1-80ng/ml.Ngati ipitilira kuchuluka kwake, yambitsani <1ng/ml, kapena> 80 ng/ml.
Chiyambireni kukhazikitsidwa kwake, fakitale yathu yakhala ikupanga zinthu zapamwamba kwambiri padziko lonse lapansi ndikutsatira mfundoyi
za khalidwe poyamba.Zogulitsa zathu zapeza mbiri yabwino pamsika komanso kukhulupirika pakati pa makasitomala atsopano ndi akale..