Canine Health Markers Kuphatikiza Kuzindikira (zinthu 5-6)

【Cholinga choyesera】
Canine pancreatic lipase (cPL) : Canine kapamba ndi matenda olowa m'matumbo a kapamba.Nthawi zambiri, imatha kugawidwa kukhala pachimake kapamba komanso pancreatitis yosatha.Pancreatic neutrophil infiltration, pancreatic necrosis, peripancreatic fat necrosis, edema ndi kuvulala kumatha kuwoneka pachimake kapamba.Pancreatic fibrosis ndi atrophy imatha kuwoneka mu kapamba osatha.Poyerekeza ndi kapamba pachimake, kapamba osatha siwowopsa, koma pafupipafupi.Agalu akadwala kapamba, kapamba amawonongeka, ndipo kuchuluka kwa pancreatic lipase m'magazi kumawonjezeka kwambiri.Pakadali pano, pancreatic lipase ndi chimodzi mwazizindikiro zabwino kwambiri zozindikiritsira kapamba mwa agalu.
Cholyglycine (CG) ndi imodzi mwa ma conjugated cholic acid omwe amapangidwa ndi kuphatikiza kwa cholic acid ndi glycine.Glycocholic acid ndi gawo lofunikira kwambiri la bile acid mu seramu panthawi yoyembekezera.Maselo a chiwindi atawonongeka, kutengeka kwa CG ndi maselo a chiwindi kunachepa, zomwe zinachititsa kuwonjezeka kwa CG m'magazi.Mu cholestasis, kutuluka kwa cholic acid ndi chiwindi kumasokonekera, ndipo zomwe zili mu CG yobwerera m'magazi zimachulukitsidwa, zomwe zimawonjezera kuchuluka kwa CG m'magazi.
Cystatin C ndi imodzi mwa mapuloteni a cystatin.Pakadali pano, Cys C ndi chinthu chokhazikika chomwe chimakwaniritsa zofunikira za cholembera cha GFR.Ndilo cholozera chodziwika bwino chowunika ntchito yaimpso ya canine.
N-terminal pro-brain natriuretic peptide (Canine NT-proBNP) ndi chinthu chopangidwa ndi cardiomyocytes mu Canine ventricle ndipo chingagwiritsidwe ntchito ngati cholozera chozindikiritsa cha kulephera kwa mtima kofanana.The ndende cNT-proBNP mu magazi correlates ndi kuopsa kwa matenda.Choncho, NT-proBNP sangakhoze kokha kupenda kuopsa kwa pachimake ndi aakulu mtima kulephera, komanso ntchito monga chizindikiro cha matenda ake.
Canine allergen total IgE (cTIgE) :IgE ndi mtundu wa immunoglobulin (Ig) wokhala ndi molekyulu yolemera 188kD komanso yotsika kwambiri mu seramu.Nthawi zambiri ntchito matenda a thupi lawo siligwirizana.Kuphatikiza apo, ingathandizenso pakuzindikira matenda a parasitic ndi ma myeloma angapo.1. Matupi awo sagwirizana: pamene ziwengo zimachitika, zimabweretsa kuwonjezeka kwa allergen lgE.Kukwera kwa allergener LgE, m'pamenenso kusagwirizana ndizovuta kwambiri.2. Matenda a tizilombo: chiweto chikadwala ndi tizilombo toyambitsa matenda, allergen LgE imathanso kuwonjezeka, yomwe nthawi zambiri imakhala yokhudzana ndi kuchepa pang'ono chifukwa cha mapuloteni a tizilombo.Kuphatikiza apo, kupezeka kwa khansa kungapangitsenso kukweza kwa IgE yonse.

【 Mfundo yodziwira】
Mankhwalawa amagwiritsa ntchito fluorescence immunochromatography kuti azindikire kuchuluka kwa cPL/CG/cCysC/cNT-proBNP/cTIgE zomwe zili m'magazi a canine.Mfundo yaikulu ndi yakuti nembanemba ya nitrocellulose imakhala ndi mizere ya T ndi C, ndipo mzere wa T umakutidwa ndi antibody omwe amazindikira makamaka antigen.Pad yomangiriza imapopera ndi fulorosenti ina ya nanomaterial yotchedwa antibody b yomwe imatha kuzindikira antigen.Antibody mu chitsanzocho imamangiriza ku nanomaterial yolembedwa kuti antibody b kupanga zovuta, zomwe kenako zimamanga ku T-line antibody A kupanga masangweji.Kuwala kosangalatsa kukayatsidwa, nanomaterial imatulutsa ma siginecha a fulorosenti.Kuchuluka kwa chizindikirocho kunali kogwirizana bwino ndi ndende ya antigen mu chitsanzo.